Multiplex imaging of apoptosis. U2OS cells were treated with 30 μM etoposide for 18 hr to induce apo
Multiplex imaging of apoptosis. U2OS cells were treated with 30 μM etoposide for 18 hr to induce apoptosis. The treated cells were stained first with 7.5 μM CellEvent Caspase-3/7 Green detection reagent (green fluorescence, C10423) to detect apoptosis and Hoechst 33342 nucleic acid stain (blue fluorescence, H3570) to label nuclei, and then with 150 nM MitoTracker Deep Red FM (pink fluorescence, M22426) to label mitochondria. Following fixation and permeabilization, actin was labeled with Alexa Fluor 546 phalloidin (orange fluorescence, A22283). -- source link
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