The microplates can be classified like this!According to the color, it is divided into transparent,
The microplates can be classified like this!According to the color, it is divided into transparent, black and white.The transparent one is the most commonly used and is used for the most general enzyme-linked immunoassays. Compared with transparent boards, there are opaque boards used for luminescence detection, generally black and white. The black ELISA plate itself absorbs light, so its signal is much lower than that of the white plate, so it is generally used to detect stronger light, such as fluorescence detection. The white ELISA plate is used for weak light detection, and is often used for general chemiluminescence. In addition, the black ELISA plate can also reduce the problems caused by non-specific reactions. Some customers may ask whether luminescence detection is possible with a general ELISA plate. The answer is no, because generally the light emitted from the chemiluminescence reaction is isotropic, that is to say, it is emitted equally in all directions. . If you use a transparent board, the light will not only diverge from the vertical direction, but also from the horizontal direction. It will easily pass through the gaps between the holes and the hole walls. In this case, the influence between adjacent wells will be great when the light is strong, so it is not possible to use a transparent ELISA plate for chemiluminescence experiments. -- source link
#microplate#elisa plate#immunoassay